Supplementary Figure 9: Comparison of light-induced MYOD1 and IL1RN activation efficiency among various sgRNAs targeting different sites in the promoter regions. | Nature Methods

Supplementary Figure 9: Comparison of light-induced MYOD1 and IL1RN activation efficiency among various sgRNAs targeting different sites in the promoter regions.

From: CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Supplementary Figure 9

Light-induced upregulation of MYOD1 (a) and IL1R2 (b) in HEK293T cells using Split-CPTS2.0, CPTS2.0, CPTS and SAM with an indicated single sgRNA. Maps show the sgRNA targeting sites in each human locus. Data are expressed as relative mRNA amount to the negative control transfected with empty vector in the dark and represented as mean ± s.e.m. (n=4 from two individual experiments with two cell culture replicates).

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