Supplementary Figure 7: Spatial surrogate reporter activation by Split-CPTS2.0. | Nature Methods

Supplementary Figure 7: Spatial surrogate reporter activation by Split-CPTS2.0.

From: CRISPR–Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Supplementary Figure 7

(a) Schematic of GAL4-UAS mCherry reporter. (b) Light-induced mCherry reporter activation of Split-CPTS2.0. HEK293T cells were transfected with Split-CPTS2.0, GAL4-UAS mCherry reporter and sgRNA targeting the reporter. Twenty hours post transfection, samples were incubated under dark state, global blue light illumination for 24 h. Scale bar = 2 mm. (c) Spatial gene activation by Split-CPTS2.0. Slit-patterned mCherry expression in HEK293T cells illuminated by blue light with a spatial pattern using a photomask. EGFP is used as transfection marker. The width of slit is 2.6 mm. Close-up view within white square region is also shown. Scale bar, 2 mm (zoom-out view) and 0.5 mm (close-up view), respectively. (d) Line scan intensity profile of EGFP (turquoise) and mCherry (magenta) on dashed line in c.

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