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GUIDES: sgRNA design for loss-of-function screens

Nature Methods volume 14, pages 831832 (2017) | Download Citation

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Acknowledgements

We would like to thank B. Cummings for help with GTEx data processing and the entire Sanjana laboratory for support and advice. F.Z. is supported by the NIH through NIMH (5DP1-MH100706 and 1R01-MH110049), the NSF, the New York Stem Cell Foundation, the Allen Distinguished Investigator Program through The Paul G. Allen Frontiers Group, the Simons and Vallee Foundations, the Howard Hughes Medical Institute, the Skoltech-MIT Next Generation Program, J. and P. Poitras and the Poitras Center for Affective Disorders, R. Metcalfe, and D. Cheng. N.E.S. is supported by the NIH through NHGRI (R00-HG008171) and the Sidney Kimmel Foundation.

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Affiliations

  1. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

    • Joshua A Meier
    •  & Feng Zhang
  2. McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Joshua A Meier
    •  & Feng Zhang
  3. New York Genome Center, New York, New York, USA.

    • Joshua A Meier
    •  & Neville E Sanjana
  4. Department of Biology, New York University, New York, New York, USA.

    • Joshua A Meier
    •  & Neville E Sanjana

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Competing interests

A patent has been filed relating to the described work. F.Z. is a founder and scientific advisor for Editas Medicine and a scientific advisor for Horizon Discovery.

Corresponding author

Correspondence to Neville E Sanjana.

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DOI

https://doi.org/10.1038/nmeth.4423

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