Supplementary Figure 5 : Multimodal bi-axial plots.

From: Simultaneous epitope and transcriptome measurement in single cells

Supplementary Figure 5

(a) Histograms of CLR-transformed ADT counts in CBMCs (red) and mouse control cells spiked at very low frequency (blue). Solid line shows the determined cutoff for significant ADT signal (mean of mouse values + standard deviation of mouse values). (b) Histograms of CLR-transformed ADT counts for the three antibodies-oligo conjugates in our 13 antibody pool that did not pass the mouse-derived threshold. (c) Multimodal bi-axial plots. Pairwise comparison of different CLR-transformed ADT levels in CBMCs. Upper right: CBMCs plotted with colors based on RNA clusters shown in Figure 3a. Lower left: mouse control cells (black) that were spiked at very low frequency are overlaid on the CBMCs (light grey).