Supplementary Figure 2 : Analysis of mixtures of mouse and human cells that were incubated with oligo-tagged-antibodies specific for either human or mouse cell-surface markers.

From: Simultaneous epitope and transcriptome measurement in single cells

Supplementary Figure 2

(a) Fractions of human RNA molecules compared to human ADTs for detected cell barcodes in the Drop-seq species mixing experiment. Each point represents one cell barcode (i.e., droplet containing one or more cells). (b-e) CITE-seq of human and mouse mixing experiment repeated on a commercially available system from 10x Genomics. (b) Quantification of the number of human and mouse transcripts associating to each cell barcode. Each green point indicates a cell barcode (i.e. droplet containing one or more cells) from which we measured >90% human transcripts; each red point indicates a cell barcode with >90% mouse transcripts. Blue points indicate cell barcodes (i.e. droplets) from which we observed a mixture of human and mouse transcripts. (c) Quantification of antibody tags (ADTs) associated with each cell barcode. Points are colored based on species classifications using transcripts in panel b. (d) Quantification of human, mouse or mixed-cell barcodes based on RNA transcripts, or ADTs, detected in 10x Genomics workflows. (e) Fractions of human RNA molecules compared to human ADTs for detected cell barcodes. Each point represents one cell barcode (i.e., droplet containing one or more cells).