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Digenome-seq web tool for profiling CRISPR specificity

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Figure 1: Digenome-seq overview.

References

  1. Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–243, 1, 243 (2015).

    Article  CAS  Google Scholar 

  2. Kim, D., Kim, S., Kim, S., Park, J. & Kim, J.S. Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq. Genome Res. 26, 406–415 (2016).

    Article  CAS  Google Scholar 

  3. Kim, D. et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells. Nat. Biotechnol. 34, 863–868 (2016).

    Article  CAS  Google Scholar 

  4. Aken, B.L. et al. Ensembl 2017. Nucleic Acids Res. 45, D635–D642 (2017)

    Article  CAS  Google Scholar 

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Acknowledgements

We thank M. Schlesner and R. Eils for helpful discussion. This work was supported by the Institute for Basic Science grant no. IBS-R021-D1 to J.-S.K. and Next Generation BioGreen 21 Program grant no. PJ01119201 and Korea Healthcare technology R&D Project grant no. HI16C1012 to S.B.

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Correspondence to Jin-Soo Kim or Sangsu Bae.

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S.B. and J.P. have filed a patent application based on this work.

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Park, J., Childs, L., Kim, D. et al. Digenome-seq web tool for profiling CRISPR specificity. Nat Methods 14, 548–549 (2017). https://doi.org/10.1038/nmeth.4262

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