Supplementary Figure 8: Comparison of Unblur and MotionCor2 on a tomographic tilt series. | Nature Methods

Supplementary Figure 8: Comparison of Unblur and MotionCor2 on a tomographic tilt series.

From: MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Supplementary Figure 8

This tomographic tilt series was collected between [−60°, 60°] at every 2° from a frozen hydrated specimen of Drosophila centriole. The data collection was performed in two branches that started from −30° to 60° and then from −32° to −60° and at the magnification that gives rise to the pixel size of 4.08Å. At each angular step a movie stack of 20 sub-frames was collected at dose rate of 5e/pixel/s with frame exposure of 0.25s. Under this setup the per-frame dose is 0.075e2 and the total dose of the entire tilt series is 92e2.

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