Supplementary Figure 5: Comparison of motion correction by MotionCor2 and MotionCorr on 3D reconstruction of rat TRPV1 ion channel. | Nature Methods

Supplementary Figure 5: Comparison of motion correction by MotionCor2 and MotionCorr on 3D reconstruction of rat TRPV1 ion channel.

From: MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Supplementary Figure 5

A published dataset of frozen hydrated rat TRPV1 ion channel was reprocessed using MotionCor2. (a) Cross Correlation Coefficient of CTFFIND49 from the correction by both MotionCorr (red) and MotionCor2 (blue). (b) Histogram of cross correlation coefficients determined by using CTFFIND4 using image corrected by MotionCor2 (blue) and MotionCorr (red). The difference, which shows the amount of improvement, is shown in brown. (c) Representative 2D class averages of frozen hydrated TRPV1 particles. (d) FSC curves of 3D reconstructions determined from the same dataset after motion correction by MotionCorr (red), MotionCor2 (blue) and MotionCor2 with dose weighting (brown). (e) A representative view of the TRPV1 ion channel generated from previously published density map5. (f) The view of the same region of TRPV1 density map determined after re-process motion correction using MotionCor2. Both maps (e and f) are shown at the same normalized density level, σ = 6.

Back to article page