Commentary | Published:

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

Nature Methods volume 14, pages 228232 (2017) | Download Citation

Abstract

We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.

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Acknowledgements

This work was supported by the Fund for Scientific Spearheads of the Ghent University Hospital, Concerted Research Actions from Ghent University, Stichting tegen Kanker, Kom Op Tegen Kanker, H2020/COST ME-HaD, PhD (J.V.D.) and postdoctoral (A.H., P.M.) positions from Fund for Scientific Research Flanders (FWO) and Krediet aan Navorsers (A.H.) from FWO. This manuscript does not necessarily represent the views of organizations of which authors may be members.

Author information

Affiliations

  1. Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium.

    • Jan Van Deun
    • , Tine Baetens
    • , Clara Casert
    • , Delphine Daveloose
    • , Victoria Depoorter
    • , Bert Dhondt
    • , Edward Geeurickx
    • , Lien Lippens
    • , Quentin Rousseau
    • , Joeri Tulkens
    • , Glenn Vergauwen
    • , Olivier De Wever
    •  & An Hendrix
  2. Center for Medical Genetics, Cancer Research Institute Ghent (CRIG), Bioinformatics Institute Ghent (BIG), Ghent University, Ghent, Belgium.

    • Pieter Mestdagh
    • , Jasper Anckaert
    • , Farzaneh Ghazavi
    • , Hetty Helsmoortel
    • , Carina Leonelli
    • , Gloria Milani
    • , Liselot M Mus
    • , Alan Van Goethem
    • , Nadine Van Roy
    • , Suzanne Vanhauwaert
    • , Annelynn Wallaert
    •  & Jo Vandesompele
  3. Cell Death Research & Therapy (CDRT) Lab, KU Leuven University of Leuven, Leuven, Belgium.

    • Patrizia Agostinis
    • , Seyma Demirsoy
    • , Aleksandra Dudek
    • , Abhishek D Garg
    • , Shaun Martin
    • , Erminia Romano
    •  & Alexander R Van Vliet
  4. Department of Biomedical Molecular Biology, Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium.

    • Özden Akay
    • , Geert Berx
    • , Eva De Smedt
    • , Bieke Soen
    •  & Niels Vandamme
  5. Molecular and Cellular Oncology Lab, Inflammation Research Center, VIB, Ghent, Belgium.

    • Özden Akay
    • , Geert Berx
    • , Eva De Smedt
    • , Bieke Soen
    •  & Niels Vandamme
  6. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.

    • Sushma Anand
    • , Stephanie Boukouris
    • , Lesley Cheng
    • , Andrew F Hill
    • , Hina Kalra
    • , Suresh Mathivanan
    • , Monisha Samuel
    •  & Benjamin Scicluna
  7. Unidad de Investigación, Hospital Santa Cristina Instituto de Investigación Sanitaria Princesa C, Madrid, Spain.

    • Zoraida Andreu Martinez
  8. Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Rommelaere Campus, Ghent, Belgium.

    • Els Beghein
    • , Laurence Bertier
    • , Jan Gettemans
    • , Anneleen Steels
    •  & Isabel Van Audenhove
  9. Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.

    • Janneke Boere
    • , Tom A P Driedonks
    • , Tom Groot Kormelink
    • , Esther N M Nolte-'t Hoen
    • , Susanne van der Grein
    • , Martijn J van Herwijnen
    • , Marca Wauben
    •  & Marijke I Zonneveld
  10. Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Essen, Germany.

    • Michel Bremer
    •  & Bernd Giebel
  11. Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich (TUM) Weihenstephan, Freising, Germany.

    • Dominik Buschmann
    •  & Michael W Pfaffl
  12. Laboratory of Cytometry, Department of Internal Medicine, University of Michigan Medical School, University of Michigan, Ann Arbor, Michigan, USA.

    • James B Byrd
  13. Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.

    • Anna Cmoch
  14. Institute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, Kiel, Germany.

    • Abdou Elsharawy
  15. Faculty of Sciences, Division of Biochemistry, Chemistry Department, Damietta University, New Damietta City, Egypt.

    • Abdou Elsharawy
  16. UMR-1280, INRA, University of Nantes, Physiologie des Adaptations Nutritionnelles, Nantes, France.

    • Ilaria Floris
  17. Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada.

    • Ilaria Floris
  18. Research Unit Gene Vectors, Helmholtz Zentrum München, Munich, Germany.

    • Kathrin Gärtner
  19. German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.

    • Kathrin Gärtner
  20. Department of Molecular and Comparative Pathobiology and Department of Neurology, The Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.

    • Grace Hancock
    • , Dillon C Muth
    •  & Kenneth W Witwer
  21. INSERM U1109, MN3T, Strasbourg, France.

    • Vincent Hyenne
  22. LabEx Medalis, Université de Strasbourg, Strasbourg, France.

    • Vincent Hyenne
  23. Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.

    • Vincent Hyenne
  24. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

    • David Kim
    • , Yaxuan Liang
    • , Prabhu Mathiyalagan
    •  & Susmita Sahoo
  25. Institut Curie, PSL Research University, INSERM U932, Paris, France.

    • Joanna Kowal
    • , Bjarke Primdal-Bengtson
    •  & Clotilde Théry
  26. Department of Thoracic and Cardiovascular Surgery, University Hospital RWTH Aachen, Germany.

    • Sandra Kraemer
  27. Institute of Human Genetics, Saarland University, Homburg, Germany.

    • Petra Leidinger
  28. German Center for Neurodegenerative Diseases Bonn (DZNE e.V.), Bonn, Germany.

    • Shu Liu
  29. Institut Curie, PSL Research University, CNRS, UMR 144, Paris, France.

    • Alessandra Lo Cicero
    • , Roberta Palmulli
    • , Guillaume Van Niel
    •  & Frederik Verweij
  30. Université Côte d'Azur, Inserm, CNRS, iBV, Nice, France.

    • Támas Matusek
  31. REMAR-IVECAT Group, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, Autonomous University of Barcelona, Barcelona, Spain.

    • Marta Monguió-Tortajada
  32. Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.

    • Andrea Németh
  33. School of Pharmacy and Pharmaceutical Sciences and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland.

    • Lorraine O'Driscoll
  34. Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.

    • Natalia Sampaio
  35. Laboratory of Lipid Metabolism and Cancer, Department of Oncology, LKI – Leuven Cancer Institute, KU Leuven University of Leuven, Leuven, Belgium.

    • Johannes V Swinnen
  36. Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences and Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Finland.

    • Maarit Takatalo
  37. Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan.

    • Maarit Takatalo
    •  & Safia Thaminy
  38. Inflammation Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.

    • Andrew D Foers
  39. Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia.

    • Andrew D Foers

Consortia

  1. EV-TRACK Consortium

Authors

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  2. Search for Pieter Mestdagh in:

  3. Search for Patrizia Agostinis in:

  4. Search for Özden Akay in:

  5. Search for Sushma Anand in:

  6. Search for Jasper Anckaert in:

  7. Search for Zoraida Andreu Martinez in:

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  9. Search for Els Beghein in:

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  12. Search for Janneke Boere in:

  13. Search for Stephanie Boukouris in:

  14. Search for Michel Bremer in:

  15. Search for Dominik Buschmann in:

  16. Search for James B Byrd in:

  17. Search for Clara Casert in:

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  20. Search for Delphine Daveloose in:

  21. Search for Eva De Smedt in:

  22. Search for Seyma Demirsoy in:

  23. Search for Victoria Depoorter in:

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  25. Search for Tom A P Driedonks in:

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  30. Search for Kathrin Gärtner in:

  31. Search for Abhishek D Garg in:

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  33. Search for Jan Gettemans in:

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  39. Search for Andrew F Hill in:

  40. Search for Vincent Hyenne in:

  41. Search for Hina Kalra in:

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  50. Search for Alessandra Lo Cicero in:

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  57. Search for Liselot M Mus in:

  58. Search for Dillon C Muth in:

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  60. Search for Esther N M Nolte-'t Hoen in:

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  64. Search for Bjarke Primdal-Bengtson in:

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Contributions

J.V.D., P.M., O.D.W., J.V. and A.H. designed the study and analyzed and interpreted the data. J.V.D., P.M., P.A., G.B., J.G., B.G., A.F.H., S.M., E.N.M.N.-'t.H., L.O., M.W.P., S.S., J.V.S., C.T., G.V.N., M.W., K.W.W., O.D.W., J.V. and A.H. discussed and prepared the manuscript. J.V.D., P.M., J.A., O.D.W., J.V. and A.H. developed the online tool. All authors annotated data and approved of the final manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to An Hendrix.

Integrated supplementary information

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–6, Supplementary Tables 1–5 and Supplementary Methods

Excel files

  1. 1.

    Supplementary Table 6

    Calculation of the EV-METRIC for all EV-TRACK experiments.

About this article

Publication history

Published

DOI

https://doi.org/10.1038/nmeth.4185

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