Supplementary Figure 4: Validation of the T cell receptor gRNA library and gRNA dynamics | Nature Methods

Supplementary Figure 4: Validation of the T cell receptor gRNA library and gRNA dynamics

From: Pooled CRISPR screening with single-cell transcriptome readout

Supplementary Figure 4

a) gRNA representation in the T cell receptor (TCR) gRNA library, assessed by amplicon sequencing of the plasmid pool (top) and the gDNA of Jurkat cells at day 10 post transduction with CROPseq-Guide-Puro (bottom), both displayed as cumulative distribution plots. The fold change between the 10th and 90th percentile is highlighted as a measure of library imbalance, which expectedly increases upon transduction. b) Abundance of each gRNA shown as a heatmap. c) Scatterplots of gRNA abundance from amplicon libraries at day 10 versus the original plasmid library. Frequencies of detected gRNAs have been normalized to the evaluated reads in each experiment.

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