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Vibrio natriegens as a fast-growing host for molecular biology

Nature Methods volume 13, pages 849851 (2016) | Download Citation

Abstract

A rapidly growing bacterial host would be desirable for a range of routine applications in molecular biology and biotechnology. The bacterium Vibrio natriegens has the fastest growth rate of any known organism, with a reported doubling time of <10 min. We report the development of genetic tools and methods to engineer V. natriegens and demonstrate the advantages of using these engineered strains in common biotech processes.

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Acknowledgements

The authors thank L. Fu, C. Ludka, R. Morey, and J. Gill for assistance with genome sequencing; A. Schwartz and D. Brami for assistance with genome assembly and annotation; T. Richardson and V. Akella for providing bioinformatics analysis and support; B. Griffin and R. Monds for technical advice; and J.C. Venter, H. Smith, O. Fetzer, and T. Peterson for their support and input on the project.

Author information

Affiliations

  1. Synthetic Genomics, Inc., La Jolla, California, USA.

    • Matthew T Weinstock
    • , Eric D Hesek
    • , Christopher M Wilson
    •  & Daniel G Gibson

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Contributions

M.T.W. conceived the study; M.T.W., E.D.H., C.M.W., and D.G.G. designed experiments; M.T.W., E.D.H., and C.M.W. performed experiments; M.T.W., E.D.H., C.M.W., and D.G.G. analyzed data and wrote the paper.

Competing interests

M.T.W., E.D.H., C.M.W. and D.G.G. are employed by Synthetic Genomics, Inc. (SGI), a privately held company that funded this work. SGI has filed a provisional application with the US Patent and Trademark Office on aspects of this research.

Corresponding authors

Correspondence to Matthew T Weinstock or Daniel G Gibson.

Supplementary information

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    Supplementary Text and Figures

    Supplementary Figures 1–20, Supplementary Tables 1–4 and Supplementary Notes 1–5.

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    Supplementary Data

    Plasmid sequences.

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DOI

https://doi.org/10.1038/nmeth.3970

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