Genotyping tumor clones from single-cell data

A new statistical approach mitigates technical errors in single-cell DNA sequencing data to advance the study of tumor evolution and diversity.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Technical errors can lead to incorrect results for clonal genotypes and lineages.

References

  1. 1

    Navin, N.E. Genome Res. 25, 1499–1507 (2015).

  2. 2

    Zafar, H., Wang, Y., Nakhleh, L., Navin, N. & Chen, K. Nat. Methods 13, 505–507 (2016).

  3. 3

    Jahn, K., Kuipers, J. & Beerenwinkel, N. Genome Biol. 17, 86 (2016).

  4. 4

    Ross, E.M. & Markowetz, F. Genome Biol. 17, 69 (2016).

  5. 5

    Roth, A. et al. Nat. Methods 13, 573–576 (2016).

  6. 6

    Gawad, C., Koh, W. & Quake, S.R. Nat. Rev. Genet. 17, 175–188 (2016).

  7. 7

    Gawad, C., Koh, W. & Quake, S.R. Proc. Natl. Acad. Sci. USA 111, 17947–17952 (2014).

  8. 8

    Yuan, K., Sakoparnig, T., Markowetz, F. & Beerenwinkel, N. Genome Biol. 16, 36 (2015).

  9. 9

    McPherson, A. et al. Nat. Genet. http://dx.doi.org/10.1038/ng.3573 (2016).

  10. 10

    Navin, N.E. Genome Biol. 15, 452 (2014).

Download references

Acknowledgements

This work was supported by the Eric & Liz Lefkofsky Family Foundation, NCI (1R01CA169244-01 to N.E.N., R01CA172652 to K.C.) and the American Cancer Society (129098-RSG-16-092-01-TBG to N.E.N.). N.E.N. and K.C. are Andrew Sabin Family Fellows. The study was supported by the MD Anderson Cancer Moonshot Knowledge Gap Award and the Center for Genetics & Genomics.

Author information

Correspondence to Nicholas E Navin.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Navin, N., Chen, K. Genotyping tumor clones from single-cell data. Nat Methods 13, 555–556 (2016) doi:10.1038/nmeth.3903

Download citation

Further reading