(a) Relative cell count (left) and GR values (right) were evaluated for hTERT RPE-1 cells expressing BRAFV600E under the control of a DOX-regulated promoter 48 h after treatment with etoposide. Color intensity reflects the concentration of DOX used to stimulate BRAFV600E expression. (b) Relative cell count (left) and GR values (right) were evaluated for MCF 10A cells grown in serum-free media with different levels of EGF 59 h after treatment with etoposide. Color intensity reflects treatments with different concentrations of EGF. (c) Evaluation of relative cell count (top) and GR values (bottom) for a drug concentration close to the GR50 value (left) and computed response metrics at different time points (middle and right) as estimated from live-cell imaging of BT-20 cells exposed to one of five drugs with different mechanisms of action. Data shown are from one of two biological replicates.