Supplementary Figure 3 : Flow cytometric characterization of aTF library screening.

From: Engineering an allosteric transcription factor to respond to new ligands

Supplementary Figure 3

RFU denotes relative fluorescence units. (a) Flow cytometry histogram of representative LacI variant library (red) before and (blue) after colicin E1 negative selection. (b) Flow cytometry histogram of representative LacI variant library with no inducer molecule present (blue) or exposed to a new target inducer molecule (red).