Supplementary Figure 9 : Cross-reactivity of additional LacI variants toward three other untargeted inducers and IPTG.

From: Engineering an allosteric transcription factor to respond to new ligands

Supplementary Figure 9

For additional variants displayed in Fig. 2, a dose-response was determined for non-target ligands and IPTG. Values displayed represent the highest fold induction at any ligand concentration. Inducers are colored as follows: gentiobiose, red; fucose, green; lactitol, blue; sucralose, magenta; and IPTG, black. Variants displayed were designed for binding to (a) gentiobiose, (b) fucose, (c) lactitol, and (d) sucralose. Error bars represent standard deviation of fold induction from three biological replicates.