Supplementary Figure 8 : Comparison of gentiobiose response versus single-amino-acid substitutions found after negative selection.

From: Engineering an allosteric transcription factor to respond to new ligands

Supplementary Figure 8

Induction values for gentiobiose-responding mutants are shown in pink. The induction values show the maximum weighted fold-change of response after positive selection. The color shades outside the amino acid substitution profile denotes the location of the residue in the binding pocket, dimerization interface, DNA-binding domain or as unclassified. The black outlines indicate depletion of next-generation sequencing reads for single amino-acid substitutions. The depletion value is the log2 fold-change of reads prior to negative selection divided by the read counts after negative selection. Read counts were quantile normalized between pre- and post-selection separately for each amplicon (see Online Methods).