Supplementary Figure 7 : Comparison of conservation of amino acids and mutations found for fucose response.

From: Engineering an allosteric transcription factor to respond to new ligands

Supplementary Figure 7

Mutations conferring fucose response in LacI are shown as red outlines. (a) A set of 41 LacI orthologs were aligned and the frequency of amino acid utilization is shown in blue. (b) Five experimentally validated sequences of GalR/S known to bind fucose were aligned with E. coli LacI and shown with respect to LacI positions. Mutations at positions 79 and 273 overlap with preferentially conserved amino acids in the GalR set, shown with arrows. The highest inducer at position 291 was conserved in neither LacI or fucose-responding GalR/S.