Supplementary Figure 5 : Sequence and fold induction of the top-scoring full-length Rosetta design variants.

From: Engineering an allosteric transcription factor to respond to new ligands

Supplementary Figure 5

Induction response in relative fluorescence units (RFU) with and without ligand for WT LacI and top five scoring full-length Rosetta design variants for sucralose, lactitol and fucose. WT LacI was induced with IPTG, and the full-length Rosetta design variants were induced with their respective target ligands. The mutations in each Rosetta design variant are shown above the bar graph. All ligands were supplemented at 10 mM.