Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Widespread nuclease contamination in commonly used oxygen-scavenging systems

Nature Methods volume 12, pages 901–902 (2015)Cite this article

Subjects

To the Editor:

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Get just this article for as long as you need it

$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Nuclease contamination of oxygen-scavenging enzymes; purification and analysis of PCD.

References

  1. Rasnik, I., McKinney, S.A. & Ha, T. Nat. Methods 3, 891–893 (2006).

    Article  CAS  Google Scholar 

  2. Shi, X., Lim, J. & Ha, T. Anal. Chem. 82, 6132–6138 (2010).

    Article  CAS  Google Scholar 

  3. Aitken, C.E., Marshall, R.A. & Puglisi, J.D. Biophys. J. 94, 1826–1835 (2008).

    Article  CAS  Google Scholar 

  4. Ohlendorf, D.H., Lipscomb, J.D. & Weber, P.C. Nature 336, 403–405 (1988).

    Article  CAS  Google Scholar 

  5. Juette, M.F. et al. Curr. Opin. Chem. Biol. 20, 103–111 (2014).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank our laboratory members for their insights and helpful discussions. This work was supported by the National Research Foundation of Korea (grant 2011-0013901 to J.-B.L.) and the US National Institutes of Health (grant AI099854 to K.E.Y.; grants GM080176 and CA67007 to R.F.).

Author information

Author notes

  1. Gayan Senavirathne and Jiaquan Liu: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University Medical Center, Columbus, Ohio, USA

    Gayan Senavirathne, Jiaquan Liu, Miguel A Lopez Jr, Jeungphill Hanne, Juana Martin-Lopez, Kristine E Yoder & Richard Fishel

  2. Department of Physics, Pohang University of Science and Technology, Pohang, Korea

    Jong-Bong Lee

  3. School of Interdisciplinary Bioscience and Bioengineering, Pohang, Korea

    Jong-Bong Lee

  4. Physics Department, The Ohio State University, Columbus, Ohio, USA

    Richard Fishel

Authors
  1. Gayan Senavirathne
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiaquan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miguel A Lopez Jr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeungphill Hanne
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Juana Martin-Lopez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jong-Bong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kristine E Yoder
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Richard Fishel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Richard Fishel.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–11, Supplementary Tables 1 and 2 and Supplementary Notes 1–7 and Supplementary Methods (PDF 3436 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Senavirathne, G., Liu, J., Lopez, M. et al. Widespread nuclease contamination in commonly used oxygen-scavenging systems. Nat Methods 12, 901–902 (2015). https://doi.org/10.1038/nmeth.3588

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3588

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing