Supplementary Figure 2 : Examples of the successful registration of brains imaged pre-fixation and post-fixation.

From: Whole-brain activity mapping onto a zebrafish brain atlas

Supplementary Figure 2

Tg(Elavl3:GCaMP5G)2 fish were imaged live by 2-photon microscopy to record calcium activity. Fish were then fixed, and immunostained for pERK and tERK (not shown), and the Tg(Elavl3:GCaMP5G) transgene was re-imaged by confocal microscopy. The post-fixation image data was then registered into the anatomical stack acquired during live imaging using CMTK, and the imaging slice was re-identified using 3D cross correlation analysis. This resulted in cellular resolution overlap between the pre-and post fixation data. Shown are examples from two different fish. Scale bars, 50 μm.