Supplementary Figure 5: Global comparison of standard ChIP-seq and ChIPmentation data | Nature Methods

Supplementary Figure 5: Global comparison of standard ChIP-seq and ChIPmentation data

From: ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Supplementary Figure 5

(a) Pairwise scatterplots comparing standard ChIP-seq and ChIPmentation for H3K4me1, H3K4me3, H3K27ac, H3K27me3, and H3K36me3. (b) Peak overlap calculated as the percentage of top-X% peaks in one method that overlap with peaks in the other method. (c) Composite plot for the distribution of histone marks along all genes, shown separately for standard ChIP-seq (left) and ChIPmentation (right). Chromatin accessibility obtained by ATAC-seq is shown in black. (d) Fraction of reads in peaks (FRiP) and number of peaks called from standard ChIP-seq (left) and ChIPmentation (right) data for all sequenced libraries. Note that the sequencing depth varies between replicates (Supplementary Table 1).

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