Supplementary Figure 3: Effect of tagmentation enzyme concentration on ChIPmentation library size distributions | Nature Methods

Supplementary Figure 3: Effect of tagmentation enzyme concentration on ChIPmentation library size distributions

From: ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Supplementary Figure 3

DNA fragment size distribution of ChIPmentation libraries for H3K4me3 that were prepared with different amounts of Tn5 transposase (0.2 µl to 5 µl enzyme from the Illumina Nextera DNA library preparation kit). Fragment size distributions after reverse-crosslinking but before library enrichment are shown in red, fragment size distributions after enrichment PCR are shown in green, and fragment size distributions of the size-selected final libraries are shown in blue.

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