Supplementary Figure 1: Schematic overview of standard ChIP-seq, ChIP-tagmentation and ChIPmentation | Nature Methods

Supplementary Figure 1: Schematic overview of standard ChIP-seq, ChIP-tagmentation and ChIPmentation

From: ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

Supplementary Figure 1

Workflow of ChIPmentation as compared to standard ChIP-seq and ChIP-tagmentation with purified ChIP DNA. All three protocols start by fixing cells with formaldehyde, followed by cell lysis, sonication of chromatin, and immunoprecipitation with a specific antibody bound to beads. For standard ChIP-seq (left), reverse-crosslinking is followed by purification of ChIP DNA, which is then subjected to library preparation in a multi-step procedure comprising end repair, purification, A-tailing, adapter ligation, and size selection. ChIP-tagmentation (center) uses purified ChIP DNA for tagmentation-based library preparation. In ChIPmentation (right), the sequencing adapters are introduced in a single step by tagmentation of bead-bound chromatin.

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