Workflow of ChIPmentation as compared to standard ChIP-seq and ChIP-tagmentation with purified ChIP DNA. All three protocols start by fixing cells with formaldehyde, followed by cell lysis, sonication of chromatin, and immunoprecipitation with a specific antibody bound to beads. For standard ChIP-seq (left), reverse-crosslinking is followed by purification of ChIP DNA, which is then subjected to library preparation in a multi-step procedure comprising end repair, purification, A-tailing, adapter ligation, and size selection. ChIP-tagmentation (center) uses purified ChIP DNA for tagmentation-based library preparation. In ChIPmentation (right), the sequencing adapters are introduced in a single step by tagmentation of bead-bound chromatin.