We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm2) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.
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The authors thank M.L. McKee for help with electron microscopy, as well as M. Liong and H. Shao for advice on magnetic assay protocols. This work was supported in part by US National Institutes of Health grants R01HL113156 (H.L.) and U54-CA119349 (R.W.) as well as the US Defense Advanced Research Projects Agency (DARPA) QuASAR program and DARPA SBIR contract W31P4Q-13-C-0064.
M.D.L. and R.L.W. are cofounders of Quantum Diamond Technologies, Inc. (QDTI). M.D.L., R.L.W. and A.Y. all serve on the Scientific Advisory Board of QDTI and have financial interests in QDTI. R.W. has financial interest in T2Biosystems. R.W.'s interests are reviewed and managed by MGH and Partners HealthCare in accordance with their conflict of interest policies.
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Glenn, D., Lee, K., Park, H. et al. Single-cell magnetic imaging using a quantum diamond microscope. Nat Methods 12, 736–738 (2015). https://doi.org/10.1038/nmeth.3449
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