Supplementary Figure 3: Optimizing Cas9 and sgRNA concentrations for electrical-synapse phenotypes. | Nature Methods

Supplementary Figure 3: Optimizing Cas9 and sgRNA concentrations for electrical-synapse phenotypes.

From: Rapid reverse genetic screening using CRISPR in zebrafish

Supplementary Figure 3

(a) Quantitation of mosaic electrical-synapse loss in injected embryos for varying amounts of gjd1a sgRNA injected with 1,200 pg of Cas9-encoding mRNA. (b) Quantitation of toxicity seen in injected embryos for varying amounts of gjd1a sgRNA injected with 1,200 pg of Cas9-encoding mRNA. In a, N = 24 embryos for each bar. In b, N > 86 embryos for each point.

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