An engineered dCas9–guide RNA complex containing three different transcriptional activation domains achieves consistently high expression of endogenous genes in a genome-wide screen.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Konermann, S. et al. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature doi:10.1038/nature14136 (10 December 2014).
Rights and permissions
About this article
Cite this article
Rusk, N. CRISPR gain-of-function screens. Nat Methods 12, 102–103 (2015). https://doi.org/10.1038/nmeth.3274
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3274
