Supplementary Figure 12: Calibration of the crowding sensor in the microscope with Ficoll PM70. | Nature Methods

Supplementary Figure 12: Calibration of the crowding sensor in the microscope with Ficoll PM70.

From: A sensor for quantification of macromolecular crowding in living cells

Supplementary Figure 12

This calibration applies to the microscope settings employed for the E. coli experiments. Each data point is an average over 9 measurements; the error bars represent the standard deviations. The data fit mCitrine/mCerulean = 7.08 × 10-3 × [% w/w Ficoll] + 0.910, with R2 = 0.99. Conditions: 10 mM NaPi, 100 mM NaCl, 2 mg/ml BSA, pH 7.4.

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