Developments in electrical and optical recording technology are scaling up the size of neuronal populations that can be monitored simultaneously. Light-sheet imaging is rapidly gaining traction as a method for optically interrogating activity in large networks and presents both opportunities and challenges for understanding circuit function.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams
Scientific Reports Open Access 22 May 2020
-
4-dimensional functional profiling in the convulsant-treated larval zebrafish brain
Scientific Reports Open Access 26 July 2017
-
Microfluidic neurite guidance to study structure-function relationships in topologically-complex population-based neural networks
Scientific Reports Open Access 22 June 2016
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Siedentopf, H. & Zsigmondy, R. Ann. Phys. 315, 1–39 (1902).
Söderbaum, H.G. Award ceremony speech. Nobelprize.orghttp://www.nobelprize.org/nobel_prizes/chemistry/laureates/1925/press.html (1926).
Voie, A.H., Burns, D.H. & Spelman, F.A. J. Microsc. 170, 229–236 (1993).
Fuchs, E., Jaffe, J., Long, R. & Azam, F. Opt. Express 10, 145–154 (2002).
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).
Holekamp, T.F., Turaga, D. & Holy, T.E. Neuron 57, 661–672 (2008).
Ahrens, M.B., Orger, M.B., Robson, D.N., Li, J.M. & Keller, P.J. Nat. Methods 10, 413–420 (2013).
Panier, T. et al. Front. Neural Circuits 7, 65 (2013).
Vladimirov, N. et al. Nat. Methods 11, 883–884 (2014).
Hammen, G.F., Turaga, D., Holy, T.E. & Meeks, J.P. Nat. Neurosci. 17, 953–961 (2014).
Tomer, R., Khairy, K., Amat, F. & Keller, P.J. Nat. Methods 9, 755–763 (2012).
Krzic, U., Gunther, S., Saunders, T.E., Streichan, S.J. & Hufnagel, L. Nat. Methods 9, 730–733 (2012).
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M. & Fraser, S.E. Nat. Methods 8, 757–760 (2011).
Cunningham, J.P. & Yu, B.M. Nat. Neurosci. 17, 1500–1509 (2014).
Fox, M.D. & Raichle, M.E. Nat. Rev. Neurosci. 8, 700–711 (2007).
Pillow, J.W. et al. Nature 454, 995–999 (2008).
Freeman, J. et al. Nat. Methods 11, 941–950 (2014).
Burns, R., Vogelstein, J.T. & Szalay, A.S. Neuron 83, 1249–1252 (2014).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Keller, P., Ahrens, M. & Freeman, J. Light-sheet imaging for systems neuroscience. Nat Methods 12, 27–29 (2015). https://doi.org/10.1038/nmeth.3214
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3214
This article is cited by
-
Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams
Scientific Reports (2020)
-
Evaluating the potential of using quantum dots for monitoring electrical signals in neurons
Nature Nanotechnology (2018)
-
4-dimensional functional profiling in the convulsant-treated larval zebrafish brain
Scientific Reports (2017)
-
Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research
Nature Protocols (2017)
-
Improving data quality in neuronal population recordings
Nature Neuroscience (2016)
