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PSFj: know your fluorescence microscope

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Figure 1: Analysis of microscope performance using PSFj.


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We thank H. Lorenz and C. Hoerth from the Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH) imaging facility, and F. Bestvater and D. Krunic from the Deutsches Krebsforschungszentrum (DKFZ) light microscopy facility, for assistance in acquiring some of the image stacks, and a number of friends for testing PSFj and providing expert feedback. This work was supported in parts by a grant from the German Research Foundation (DFG), Sonderforschungsbereich (SFB) 1036 (TP10).

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Correspondence to Michael Knop.

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Supplementary text and figures

Supplementary Figures 1 and 2, Supplementary Tables 1–4, Supplementary Note and Supplementary Methods (PDF 3426 kb)

Supplementary Software

PSFj software and manual (ZIP 44135 kb)

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Theer, P., Mongis, C. & Knop, M. PSFj: know your fluorescence microscope. Nat Methods 11, 981–982 (2014).

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