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PSFj: know your fluorescence microscope

Nature Methods volume 11, pages 981–982 (2014)Cite this article

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Figure 1: Analysis of microscope performance using PSFj.

References

  1. Cole, R.W., Jinadasa, T. & Brown, C.M. Nat. Protoc. 6, 1929–1941 (2011).

    Article  CAS  Google Scholar 

  2. Inoué, S. in Handbook of Biological Confocal Microscopy 3rd edn. (ed. Pawley, J.B.) 1–16 (Springer, 2006).

    Book  Google Scholar 

  3. Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. Nat. Methods 9, 671–675 (2012).

    Article  CAS  Google Scholar 

  4. Edelstein, A., Amodaj, N., Hoover, K., Vale, R. & Stuurman, N. Curr. Protoc. Mol. Biol. 92, 14.20 (2010). Neuronal morphometry directly from bitmap images

    Google Scholar 

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Acknowledgements

We thank H. Lorenz and C. Hoerth from the Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH) imaging facility, and F. Bestvater and D. Krunic from the Deutsches Krebsforschungszentrum (DKFZ) light microscopy facility, for assistance in acquiring some of the image stacks, and a number of friends for testing PSFj and providing expert feedback. This work was supported in parts by a grant from the German Research Foundation (DFG), Sonderforschungsbereich (SFB) 1036 (TP10).

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Authors and Affiliations

  1. Zentrum für Molekulare Biologie, Universität Heidelberg, Heidelberg, Germany

    Patrick Theer, Cyril Mongis & Michael Knop

  2. Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Patrick Theer & Michael Knop

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  1. Patrick Theer
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  2. Cyril Mongis
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  3. Michael Knop
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Correspondence to Michael Knop.

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Supplementary information

Supplementary text and figures

Supplementary Figures 1 and 2, Supplementary Tables 1–4, Supplementary Note and Supplementary Methods (PDF 3426 kb)

Supplementary Software

PSFj software and manual (ZIP 44135 kb)

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Theer, P., Mongis, C. & Knop, M. PSFj: know your fluorescence microscope. Nat Methods 11, 981–982 (2014). https://doi.org/10.1038/nmeth.3102

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