PSFj: know your fluorescence microscope

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Analysis of microscope performance using PSFj.


  1. 1

    Cole, R.W., Jinadasa, T. & Brown, C.M. Nat. Protoc. 6, 1929–1941 (2011).

    CAS  Article  Google Scholar 

  2. 2

    Inoué, S. in Handbook of Biological Confocal Microscopy 3rd edn. (ed. Pawley, J.B.) 1–16 (Springer, 2006).

    Google Scholar 

  3. 3

    Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. Nat. Methods 9, 671–675 (2012).

    CAS  Article  Google Scholar 

  4. 4

    Edelstein, A., Amodaj, N., Hoover, K., Vale, R. & Stuurman, N. Curr. Protoc. Mol. Biol. 92, 14.20 (2010). Neuronal morphometry directly from bitmap images

    Google Scholar 

Download references


We thank H. Lorenz and C. Hoerth from the Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH) imaging facility, and F. Bestvater and D. Krunic from the Deutsches Krebsforschungszentrum (DKFZ) light microscopy facility, for assistance in acquiring some of the image stacks, and a number of friends for testing PSFj and providing expert feedback. This work was supported in parts by a grant from the German Research Foundation (DFG), Sonderforschungsbereich (SFB) 1036 (TP10).

Author information



Corresponding author

Correspondence to Michael Knop.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary text and figures

Supplementary Figures 1 and 2, Supplementary Tables 1–4, Supplementary Note and Supplementary Methods (PDF 3426 kb)

Supplementary Software

PSFj software and manual (ZIP 44135 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Theer, P., Mongis, C. & Knop, M. PSFj: know your fluorescence microscope. Nat Methods 11, 981–982 (2014).

Download citation

Further reading


Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing