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A critique of methods for temperature imaging in single cells

Nature Methods volume 11, pages 899–901 (2014)Cite this article

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We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

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Figure 1: Temperature mapping in living cells.

References

  1. Kortmann, J. & Narberhaus, F. Nat. Rev. Microbiol. 10, 255–265 (2012).

    Article  CAS  Google Scholar 

  2. Knight, M.R. & Knight, H. New Phytol. 195, 737–751 (2012).

    Article  CAS  Google Scholar 

  3. Zohar, O. et al. Biophys. J. 74, 82–89 (1998).

    Article  CAS  Google Scholar 

  4. Zeeb, V., Suzuki, M. & Ishiwata, S. Neurosci. Methods 139, 69–77 (2004).

    Article  Google Scholar 

  5. Suzuki, M., Tseeb, V., Oyama, K. & Ishiwatwa, S. Biophys. J. 92, L46–L48 (2007).

    Article  CAS  Google Scholar 

  6. Gota, C., Okabe, K., Funatsu, T., Harada, Y. & Uchiyama, S. J. Am. Chem. Soc. 131, 2766–2767 (2009).

    Article  CAS  Google Scholar 

  7. Martinez Maestro, L. et al. Nano Lett. 10, 5109–5115 (2010).

    Article  Google Scholar 

  8. Vetrone, F. et al. ACS Nano 4, 3254–3258 (2010).

    Article  CAS  Google Scholar 

  9. McCabe, K.M., Lacherndo, E.J., Albino-Flores, I., Sheehan, E. & Hernandez, M. Appl. Environ. Microbiol. 77, 2863–2868 (2011).

    Article  CAS  Google Scholar 

  10. Yang, J., Yang, H. & Lin, L. ACS Nano 5, 5067–5071 (2011).

    Article  CAS  Google Scholar 

  11. Wang, C. et al. Cell Res. 21, 1517–1519 (2011).

    Article  Google Scholar 

  12. Okabe, K. et al. Nat. Commun. 3, 705 (2012).

    Article  Google Scholar 

  13. Donner, J., Thompson, S.A., Kreuzer, M.P., Baffou, G. & Quidant, R. Nano Lett. 12, 2107–2111 (2012).

    Article  CAS  Google Scholar 

  14. Kucsko, G. et al. Nature 500, 54–58 (2013).

    Article  CAS  Google Scholar 

  15. Shang, L., Stockmar, F., Azadfar, N. & Nienhaus, G.U. Angew. Chem. Int. Ed. 52, 11154–11157 (2013).

    Article  CAS  Google Scholar 

  16. Tsuji, T., Yoshida, S., Yoshida, A. & Uchiyama, S. Anal. Chem. 85, 9815–9823 (2013).

    Article  CAS  Google Scholar 

  17. Kiyonaka, S. et al. Nat. Methods 10, 1232–1238 (2013).

    Article  CAS  Google Scholar 

  18. Takei, Y. et al. ACS Nano 8, 198–206 (2014).

    Article  CAS  Google Scholar 

  19. Yang, L. et al. Mikrochim. Acta 181, 743–749 (2014).

    Article  CAS  Google Scholar 

  20. Lowell, B.B. Nature 404, 652–660 (2000).

    Article  CAS  Google Scholar 

  21. Loesberg, C., van Miltenburg, J.C. & van Wijk, R.J. Therm. Biol. 7, 209–213 (1982).

    Article  Google Scholar 

  22. Johnson, M.D. et al. Proc. Natl. Acad. Sci. USA 106, 6696–6699 (2009).

    Article  CAS  Google Scholar 

  23. Zamorano, F., van de Wouwer, A. & Bastin, G.J. Biotech. 150, 497–508 (2010).

    CAS  Google Scholar 

  24. Ahn, W.S. & Antoniewicz, M.R. Metab. Eng. 13, 598–609 (2011).

    Article  CAS  Google Scholar 

  25. Ponomarev, V.V. & Migarskaya, L.B. J. Phys. Chem. 34, 1182–1183 (1960).

    Google Scholar 

  26. Inomata, N., Toda, M., Sato, M., Ishijima, A. & Ono, T. Appl. Phys. Lett. 100, 154104 (2012).

    Article  Google Scholar 

  27. Behjousiar, A., Kontoravdi, C. & Polizzi, K.M. PLoS ONE 7, e34512 (2012).

    Article  CAS  Google Scholar 

  28. Baffou, G. & Rigneault, H. Phys. Rev. B 84, 035415 (2011).

    Article  Google Scholar 

  29. Nakano, T., Kikugawa, G. & Ohara, T. J. Chem. Phys. 133, 154705 (2010).

    Article  Google Scholar 

  30. Baffou, G. et al. ACS Nano 7, 6478–6488 (2013).

    Article  CAS  Google Scholar 

  31. Bianconi, E. et al. Ann. Hum. Biol. 40, 463–471 (2013).

    Article  Google Scholar 

Download references

Acknowledgements

We acknowledge support from the Centre National de la Recherche Scientifique (CNRS), Aix-Marseille University A*Midex (ANR-2011-IDEX-0001-02) and Agence Nationale de la Recherche (ANR) grants Tkinet (ANR-2011-BSV5-019-05), France Bio Imaging (ANR-2010-INSB-04-01) and France Life Imaging (ANR-2011-INSB-0006).

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Authors and Affiliations

  1. Guillaume Baffou and Hervé Rigneault are at the Institut Fresnel, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Centrale Marseille, Marseille, France.,

    Guillaume Baffou & Hervé Rigneault

  2. Didier Marguet is at the Centre d'Immunologie de Marseille-Luminy, Aix-Marseille Université, INSERM, CNRS, Marseille, France.,

    Didier Marguet

  3. Ludovic Jullien is at the École Normale Supérieure, Paris Sciences et Lettres Research University, Sorbonne Universités, Université Pierre et Marie Curie, Université Paris 06, CNRS, Paris, France.,

    Ludovic Jullien

Authors
  1. Guillaume Baffou
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  2. Hervé Rigneault
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  3. Didier Marguet
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  4. Ludovic Jullien
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Correspondence to Guillaume Baffou.

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Baffou, G., Rigneault, H., Marguet, D. et al. A critique of methods for temperature imaging in single cells. Nat Methods 11, 899–901 (2014). https://doi.org/10.1038/nmeth.3073

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