A critique of methods for temperature imaging in single cells

We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Temperature mapping in living cells.

References

  1. 1

    Kortmann, J. & Narberhaus, F. Nat. Rev. Microbiol. 10, 255–265 (2012).

  2. 2

    Knight, M.R. & Knight, H. New Phytol. 195, 737–751 (2012).

  3. 3

    Zohar, O. et al. Biophys. J. 74, 82–89 (1998).

  4. 4

    Zeeb, V., Suzuki, M. & Ishiwata, S. Neurosci. Methods 139, 69–77 (2004).

  5. 5

    Suzuki, M., Tseeb, V., Oyama, K. & Ishiwatwa, S. Biophys. J. 92, L46–L48 (2007).

  6. 6

    Gota, C., Okabe, K., Funatsu, T., Harada, Y. & Uchiyama, S. J. Am. Chem. Soc. 131, 2766–2767 (2009).

  7. 7

    Martinez Maestro, L. et al. Nano Lett. 10, 5109–5115 (2010).

  8. 8

    Vetrone, F. et al. ACS Nano 4, 3254–3258 (2010).

  9. 9

    McCabe, K.M., Lacherndo, E.J., Albino-Flores, I., Sheehan, E. & Hernandez, M. Appl. Environ. Microbiol. 77, 2863–2868 (2011).

  10. 10

    Yang, J., Yang, H. & Lin, L. ACS Nano 5, 5067–5071 (2011).

  11. 11

    Wang, C. et al. Cell Res. 21, 1517–1519 (2011).

  12. 12

    Okabe, K. et al. Nat. Commun. 3, 705 (2012).

  13. 13

    Donner, J., Thompson, S.A., Kreuzer, M.P., Baffou, G. & Quidant, R. Nano Lett. 12, 2107–2111 (2012).

  14. 14

    Kucsko, G. et al. Nature 500, 54–58 (2013).

  15. 15

    Shang, L., Stockmar, F., Azadfar, N. & Nienhaus, G.U. Angew. Chem. Int. Ed. 52, 11154–11157 (2013).

  16. 16

    Tsuji, T., Yoshida, S., Yoshida, A. & Uchiyama, S. Anal. Chem. 85, 9815–9823 (2013).

  17. 17

    Kiyonaka, S. et al. Nat. Methods 10, 1232–1238 (2013).

  18. 18

    Takei, Y. et al. ACS Nano 8, 198–206 (2014).

  19. 19

    Yang, L. et al. Mikrochim. Acta 181, 743–749 (2014).

  20. 20

    Lowell, B.B. Nature 404, 652–660 (2000).

  21. 21

    Loesberg, C., van Miltenburg, J.C. & van Wijk, R.J. Therm. Biol. 7, 209–213 (1982).

  22. 22

    Johnson, M.D. et al. Proc. Natl. Acad. Sci. USA 106, 6696–6699 (2009).

  23. 23

    Zamorano, F., van de Wouwer, A. & Bastin, G.J. Biotech. 150, 497–508 (2010).

  24. 24

    Ahn, W.S. & Antoniewicz, M.R. Metab. Eng. 13, 598–609 (2011).

  25. 25

    Ponomarev, V.V. & Migarskaya, L.B. J. Phys. Chem. 34, 1182–1183 (1960).

  26. 26

    Inomata, N., Toda, M., Sato, M., Ishijima, A. & Ono, T. Appl. Phys. Lett. 100, 154104 (2012).

  27. 27

    Behjousiar, A., Kontoravdi, C. & Polizzi, K.M. PLoS ONE 7, e34512 (2012).

  28. 28

    Baffou, G. & Rigneault, H. Phys. Rev. B 84, 035415 (2011).

  29. 29

    Nakano, T., Kikugawa, G. & Ohara, T. J. Chem. Phys. 133, 154705 (2010).

  30. 30

    Baffou, G. et al. ACS Nano 7, 6478–6488 (2013).

  31. 31

    Bianconi, E. et al. Ann. Hum. Biol. 40, 463–471 (2013).

Download references

Acknowledgements

We acknowledge support from the Centre National de la Recherche Scientifique (CNRS), Aix-Marseille University A*Midex (ANR-2011-IDEX-0001-02) and Agence Nationale de la Recherche (ANR) grants Tkinet (ANR-2011-BSV5-019-05), France Bio Imaging (ANR-2010-INSB-04-01) and France Life Imaging (ANR-2011-INSB-0006).

Author information

Correspondence to Guillaume Baffou.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Baffou, G., Rigneault, H., Marguet, D. et al. A critique of methods for temperature imaging in single cells. Nat Methods 11, 899–901 (2014). https://doi.org/10.1038/nmeth.3073

Download citation

Further reading