Supplementary Figure 2: Independent experiment to reproduce kahrp locus editing in 3D7 parasites. | Nature Methods

Supplementary Figure 2: Independent experiment to reproduce kahrp locus editing in 3D7 parasites.

From: Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

Supplementary Figure 2

(a) RL expression levels were measured over time for no sgRNA-T (negative control) and kahrp-sgRNA-T expressing parasites that were co-transfected with pT7 RNAP-HRkahrp. (b) PCR and Western blot analyses to assess whether kahrp locus editing has occurred in the no sgRNA-T and kahrp-sgRNA-T transfections. SEM images for these parasites are shown in Figure 2f.

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