Supplementary Figure 1: In vitro assay to assess cleavage activity of selected sgRNA-T sequences. | Nature Methods

Supplementary Figure 1: In vitro assay to assess cleavage activity of selected sgRNA-T sequences.

From: Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

Supplementary Figure 1

Predicted secondary structures for kahrp- and eba-175- sgRNA-Ts with the additional T7 terminator-derived sequence are shown. Cleavage of target DNA by in vitro transcribed kahrp-, eba-175- and pUC19- (negative control) sgRNA-T was assessed in lysates derived from Cas9-expressing HEK293 cells. The numbers flanking the target DNA region used are the base pair locations in the genic locus, where position +1 corresponds to the A in the ATG start codon.

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