A combination of experimental protein analysis and imaging techniques is used to create a three-dimensional model of a synaptic bouton.
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References
Wilhelm, B.G. et al. Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science 344, 1023–1028 (2014).
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Doerr, A. Modeling the synapse. Nat Methods 11, 788–789 (2014). https://doi.org/10.1038/nmeth.3057
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DOI: https://doi.org/10.1038/nmeth.3057