Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Improved vectors and genome-wide libraries for CRISPR screening

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: CRISPR lentiviral vectors with higher functional titer.


  1. Shalem, O. et al. Science 343, 84–87 (2014).

    Article  CAS  Google Scholar 

  2. Wang, T., Wei, J.J., Sabatini, D.M. & Lander, E.S. Science 343, 80–84 (2014).

    Article  CAS  Google Scholar 

  3. Koike-Yusa, H., Li, Y., Tan, E.-P., Velasco-Herrera, M.D.C. & Yusa, K. Nat. Biotechnol. 32, 267–273 (2014).

    Article  CAS  Google Scholar 

  4. Zhou, Y. et al. Nature 509, 487–491 (2014).

    Article  CAS  Google Scholar 

  5. Hsu, P.D. et al. Nat. Biotechnol. 31, 827–832 (2013).

    Article  CAS  Google Scholar 

  6. Zhao, Y. et al. Sci. Rep. 4, 3943 (2014).

    Article  Google Scholar 

Download references


We thank the Zhang lab for support and advice. N.E.S. is supported by a postdoctoral fellowship from the Simons Center for the Social Brain at Massachusetts Institute of Technology. O.S. is supported by a postdoctoral fellowship from the Klarman Cell Observatory. F.Z. is supported by the US National Institutes of Health and NIMH through a Director's Pioneer Award (5DP1-MH100706); a Transformative R01 grant (5R01-DK097768); the Keck, Merkin, Vallee, Damon Runyon, Searle Scholars, Klingenstein and Simons Foundations; and by B. Metcalfe and J. Pauley.

Author information

Authors and Affiliations



N.E.S., O.S. and F.Z. conceived of and designed the experiments. N.E.S. and O.S. performed the experiments and analyzed the data. N.S., O.S. and F.Z. wrote the manuscript.

Corresponding author

Correspondence to Feng Zhang.

Ethics declarations

Competing interests

N.E.S., O.S. and F.Z. are on patent applications related to this work. F.Z. is a cofounder of and scientific advisor for Editas Medicine.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–3, Supplementary Methods and Supplementary Data (PDF 638 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sanjana, N., Shalem, O. & Zhang, F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods 11, 783–784 (2014).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research