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Light-sheet functional imaging in fictively behaving zebrafish

Nature Methods volume 11, pages 883884 (2014) | Download Citation

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References

  1. 1.

    et al. Nature 485, 471–477 (2012).

  2. 2.

    , , & Neuron 81, 1328–1343 (2014).

  3. 3.

    , , , & Nat. Methods 10, 413–420 (2013).

  4. 4.

    et al. Front. Neural Circuits 7, 65 (2013).

  5. 5.

    et al. Opt. Express 21, 25418–25439 (2013).

  6. 6.

    , , & Front. Neural Circuits 8, 29 (2014).

  7. 7.

    et al. Nat. Methods 11, 727–730 (2014)

  8. 8.

    et al. Nature 499, 295–300 (2013).

  9. 9.

    et al. Nat. Methods (27 July 2014).

Download references

Acknowledgements

We thank M. Coleman for writing the light-sheet microscope control software Zebrascope and for continuing support; B. Coop and T. Tabachnik for their help with hardware design; S. Narayan for help with experiments; ID&F engineers for providing help on hardware components; J. Cox, R. Larson, J. Barber, B. Brandenburg and other vivarium staff for fish husbandry; G. Ceric, V. Samalam, K. Carlisle and R. Lines for assistance with the high-performance computer cluster; D.G.C. Hildebrand and M. Koyama for discussions; and V. Jayaraman, M. Reiser and G. Murphy for comments on the manuscript. This work was supported by the Howard Hughes Medical Institute.

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  1. Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

    • Nikita Vladimirov
    • , Yu Mu
    • , Takashi Kawashima
    • , Davis V Bennett
    • , Chao-Tsung Yang
    • , Loren L Looger
    • , Philipp J Keller
    • , Jeremy Freeman
    •  & Misha B Ahrens

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Misha B Ahrens.

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Supplementary information

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    Supplementary Text and Figures

    Supplementary Figures 1–3, Supplementary Table 1 and Supplementary Note

Videos

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    Supplementary Video 1

    Whole-brain imaging during the optomotor response, top projection.

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    Supplementary Video 2

    Volumetric stack of an elavl3:H2B-GCaMP6s fish of 6 d.p.f. taken with the light-sheet microscope.

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DOI

https://doi.org/10.1038/nmeth.3040

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