Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Synthetic biology

CRISPR circuits

Nature Methods volume 11, pages 710–711 (2014)Cite this article

Subjects

CRISPR-based transcriptional activators and repressors for synthetic circuits.

When it comes to designing genetic circuits—that is, the combination of biological parts to carry out logical functions—there are several things on a synthetic biologist's wish list: easy programming, small DNA footprints and a large library of parts that can be used to build complex architectures. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 system promises to deliver on all of the above: the guide RNA (gRNA) that targets active or inactive nuclease Cas9 to its complementary DNA can be easily designed, and gRNAs are small and can be quickly created for many targets.

Your institute does not have access to this article

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

References

  1. Kiani, S., et al. CRISPR transcriptional repression devices and layered circuits in mammalian cells. Nat. Methods 11, 723–726 (2014).

    CAS  Article  Google Scholar 

  2. Nissim, L. et al. Multiplexed and programmable regulation of genetic networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol. Cell 54, 698–710 (2014).

    CAS  Article  Google Scholar 

Download references

Authors
  1. Nicole Rusk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Rusk, N. CRISPR circuits. Nat Methods 11, 710–711 (2014). https://doi.org/10.1038/nmeth.3019

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3019

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing