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Microhomology-based choice of Cas9 nuclease target sites

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Figure 1: Prediction of nuclease-induced deletion patterns associated with microhomology.


  1. 1

    Cho, S.W. et al. Nat. Biotechnol. 31, 230–232 (2013).

    CAS  Article  Google Scholar 

  2. 2

    Kim, H. & Kim, J.-S. Nat. Rev. Genet. 15, 321–334 (2014).

    CAS  Article  Google Scholar 

  3. 3

    Morton, J. et al. Proc. Natl. Acad. Sci. USA 103, 16370–16375 (2006).

    CAS  Article  Google Scholar 

  4. 4

    Qi, Y. et al. Genome Res. 23, 547–554 (2013).

    CAS  Article  Google Scholar 

  5. 5

    Sung, Y.H. et al. Nat. Biotechnol. 31, 23–24 (2013).

    CAS  Article  Google Scholar 

  6. 6

    Sung, Y.H. et al. Genome Res. 24, 125–131 (2014).

    CAS  Article  Google Scholar 

  7. 7

    Bae, S. et al. Bioinformatics 30, 1473–1475 (2014).

    CAS  Article  Google Scholar 

Download references


We thank J. Park for the help with website construction. This work was supported by the Plant Molecular Breeding Center of Next-Generation BioGreen 21 Program (PJ009081), the National Research Foundation of Korea (2013065262) and TJ Park Science Fellowship to S.B.

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Correspondence to Jin-Soo Kim.

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S.B. and J.-S.K. have filed a patent application based on this work.

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Supplementary Figures 1–6, Supplementary Tables 1–5, Supplementary Note and Supplementary Methods (PDF 1375 kb)

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Bae, S., Kweon, J., Kim, H. et al. Microhomology-based choice of Cas9 nuclease target sites. Nat Methods 11, 705–706 (2014).

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