Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Microhomology-based choice of Cas9 nuclease target sites

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Figure 1: Prediction of nuclease-induced deletion patterns associated with microhomology.

References

  1. Cho, S.W. et al. Nat. Biotechnol. 31, 230–232 (2013).

    Article  CAS  Google Scholar 

  2. Kim, H. & Kim, J.-S. Nat. Rev. Genet. 15, 321–334 (2014).

    Article  CAS  Google Scholar 

  3. Morton, J. et al. Proc. Natl. Acad. Sci. USA 103, 16370–16375 (2006).

    Article  CAS  Google Scholar 

  4. Qi, Y. et al. Genome Res. 23, 547–554 (2013).

    Article  CAS  Google Scholar 

  5. Sung, Y.H. et al. Nat. Biotechnol. 31, 23–24 (2013).

    Article  CAS  Google Scholar 

  6. Sung, Y.H. et al. Genome Res. 24, 125–131 (2014).

    Article  CAS  Google Scholar 

  7. Bae, S. et al. Bioinformatics 30, 1473–1475 (2014).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank J. Park for the help with website construction. This work was supported by the Plant Molecular Breeding Center of Next-Generation BioGreen 21 Program (PJ009081), the National Research Foundation of Korea (2013065262) and TJ Park Science Fellowship to S.B.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jin-Soo Kim.

Ethics declarations

Competing interests

S.B. and J.-S.K. have filed a patent application based on this work.

Supplementary information

Supplementary Figures, Tables and Text

Supplementary Figures 1–6, Supplementary Tables 1–5, Supplementary Note and Supplementary Methods (PDF 1375 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bae, S., Kweon, J., Kim, H. et al. Microhomology-based choice of Cas9 nuclease target sites. Nat Methods 11, 705–706 (2014). https://doi.org/10.1038/nmeth.3015

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3015

This article is cited by

Search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research