Commentary | Published:

The promise of single-cell sequencing

Nature Methods volume 11, pages 2527 (2014) | Download Citation

Individual cells of the same phenotype are commonly viewed as identical functional units of a tissue or organ. However, the deep sequencing of DNA and RNA from single cells suggests a more complex ecology of heterogeneous cell states that together produce emergent system-level function. Continuing development of high-content, real-time, multimodal single-cell measurement technologies will lead to the ultimate goal of understanding the function of an individual cell in the context of its microenvironment.

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Author information

Affiliations

  1. Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

    • James Eberwine
    •  & Jai-Yoon Sul
  2. Penn Genome Frontiers Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

    • James Eberwine
    •  & Junhyong Kim
  3. The Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.

    • Tamas Bartfai
  4. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

    • Junhyong Kim

Authors

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Competing interests

The aRNA procedure has been patented by the US PTO (#5,514,545, 5,723,290, 5,958,688 and 6,291,170) and the TIPeR procedure has a patent application pending in Pennsylvania. J.-Y.S. and J.K. are also co-inventors on the pending TIPeR patent.

Corresponding author

Correspondence to James Eberwine.

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DOI

https://doi.org/10.1038/nmeth.2769

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