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Sequencing

End-to-end RNA sequencing

Nature Methods volume 10, pages 1144–1145 (2013)Cite this article

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Long-read sequencing uncovers transcript features missed by short-read methods.

As a pioneer of deep RNA sequencing, Mike Snyder surveyed what he called the transcriptional landscape of yeast in 2008. By sequencing RNA messages many times over, it was possible to map the boundaries of expressed sequences across the yeast genome. Five years on, he and his team at Stanford University are still applying the technique to improve transcript annotation, using a technology that in some ways gives a more complete picture of transcription.

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References

  1. Sharon, D. et al. A single-molecule long-read survey of the human transcriptome. Nat. Biotechnol. 10.1038/nbt.2705 (13 October 2013).

  2. Engström, P.G. et al. Systematic evaluation of spliced alignment programs for RNA-seq data. Nat. Methods 10, 1185–1191 (2013).

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  3. Steijger, T. et al. Assessment of transcript reconstruction methods for RNA-seq. Nat. Methods 10, 1177–1184 (2013).

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Authors
  1. Tal Nawy
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Nawy, T. End-to-end RNA sequencing. Nat Methods 10, 1144–1145 (2013). https://doi.org/10.1038/nmeth.2750

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  • DOI: https://doi.org/10.1038/nmeth.2750

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