Long-read sequencing uncovers transcript features missed by short-read methods.
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Sharon, D. et al. A single-molecule long-read survey of the human transcriptome. Nat. Biotechnol. 10.1038/nbt.2705 (13 October 2013).
Engström, P.G. et al. Systematic evaluation of spliced alignment programs for RNA-seq data. Nat. Methods 10, 1185–1191 (2013).
Steijger, T. et al. Assessment of transcript reconstruction methods for RNA-seq. Nat. Methods 10, 1177–1184 (2013).
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Nawy, T. End-to-end RNA sequencing. Nat Methods 10, 1144–1145 (2013). https://doi.org/10.1038/nmeth.2750