Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray–based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.
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We dedicate this paper to T.P. We are grateful to C. Merten, M. Kaksonen, A. Galih, K. Kugler, C. Besir, M. Hsiung, M. Skruzny and the Protein Expression and Purification Core Facility for expert help and the sharing of reagents. We thank M. Mall (European Molecular Biology Laboratory) for the PKCγ and PKCδ (in the pEGFP-N3 vector). We also thank J.E.'s and other members of A.-C.G.'s groups for continuous discussions and support. This work was partially funded by the German Federal Ministry of Education and Research (BMBF; 01GS0865) in the framework of the IG-Cellular System genomics to A.-C.G. K.M. was supported by the Danish Natural Science Research Council (09-064986/FNU). A.-E.S. is supported by the European Molecular Biology Laboratory and the EU Marie Curie Actions Interdisciplinary Postdoctoral Cofunded Programme.
A.-E.S., I.V., J.E. and A.-C.G. are named on the international patent application PCT/EP2013/065256, "Giant liposome array for high-throughput lipid-protein interaction screening," which is based on the results described in this study.
Supplementary Figures 1–6, Supplementary Tables 1–3 and Supplementary Note (PDF 1596 kb)
At the initial time point (t = 0 s), a phosphate buffered saline (PBS, pH 7.4) is injected upon TAL inked with a lipid mixture composed of PC. Lipids self-assemble in liposomes in less than 3 minutes. (MOV 1082 kb)
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Saliba, AE., Vonkova, I., Ceschia, S. et al. A quantitative liposome microarray to systematically characterize protein-lipid interactions. Nat Methods 11, 47–50 (2014). https://doi.org/10.1038/nmeth.2734
Biophysical Reviews (2022)
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