Use of helper interactions to encourage weak heteromeric interactions between fluorescent protein pairs helps ensure optimal fluorescence resonance energy transfer (FRET) signals and minimizes the impact on target protein interactions.
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Jalink, K. hiFRET: some tailwind for FRET resolves weak protein interactions. Nat Methods 10, 947–948 (2013). https://doi.org/10.1038/nmeth.2652
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DOI: https://doi.org/10.1038/nmeth.2652
