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hiFRET: some tailwind for FRET resolves weak protein interactions

Use of helper interactions to encourage weak heteromeric interactions between fluorescent protein pairs helps ensure optimal fluorescence resonance energy transfer (FRET) signals and minimizes the impact on target protein interactions.

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Figure 1: hiFRET minimizes steric hindrance while yielding good FRET response.

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Correspondence to Kees Jalink.

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Jalink, K. hiFRET: some tailwind for FRET resolves weak protein interactions. Nat Methods 10, 947–948 (2013). https://doi.org/10.1038/nmeth.2652

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