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Sculpting genomes with a hammer and chisel

Nature Methods volume 10, pages 839–840 (2013)Cite this article

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With a flood of new tools for genome engineering, we can benefit from choosing the tool that best fits the job.

Genome engineering has recently seen stunning advances with the emergence of powerful site-specific nucleases. Like the early stone carvers, we are just beginning to learn how to properly use these new tools. Unlike the early carvers who could see each chip of stone, we are largely engineering in the dark. We sequence the intended mutation site to claim success, but whole-genome sequencing is still too expensive to routinely check the true fidelity of each mutational event.

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Figure 1

Marina Corral Spence

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Authors and Affiliations

  1. and the Department of Medicine, Bruce R. Conklin is at the Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA, University of California San Francisco, San Francisco, California, USA.,

    Bruce R. Conklin

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  1. Bruce R. Conklin
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Correspondence to Bruce R. Conklin.

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Conklin, B. Sculpting genomes with a hammer and chisel. Nat Methods 10, 839–840 (2013). https://doi.org/10.1038/nmeth.2608

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