We are entering a new era in neuroscience in which technological development will allow us to obtain full anatomical, high-resolution renderings of entire brain circuits and to map the activity of ever larger cellular populations as an animal performs specific behaviors. Assembling anatomical, molecular and functional maps has the potential to greatly advance our understanding of how brains work.
In this Focus, experts outline the technologies needed to obtain these maps and discuss what will be needed beyond them to understand brain function.
In a Historical Perspective, Cornelia Bargmann and Eve Marder discuss what has been learned from invertebrate circuits whose connectivity patterns are known and what will be needed beyond anatomical maps to understand brain function in other organisms. In a Commentary, Jeff Lichtman and Joshua Morgan express their views about why obtaining detailed, high-resolution structural maps should be an essential part of this endeavor. To understand the deluge of data these maps will engender once generated, Olaf Sporns argues in another Commentary that data representation and modeling will be critical.
Other experts discuss the newest technologies available for obtaining brain maps. Moritz Helmstaedter presents the state of the art and current challenges of electron microscopy–based circuit reconstruction. In three papers, the potential of using light to unveil the function and anatomy of brain circuits is presented. Karl Deisseroth and Kwanghun Chung discuss their newly developed method named CLARITY for rendering mammalian brains permeable to visible photons and molecules. Pavel Osten and Troy Margrie review light-microscopy methods available for large-scale anatomical tracing and discuss ways to integrate molecular identity, activity recording and anatomical information. In a Resource, Josh Sanes and colleagues present improved tools for mapping the mouse brain using the Brainbow technology. Finally, Michael Milham, Stan Colcombe and their colleagues review methods for functional and anatomical analysis of human brains at the macroscale.
We are pleased to acknowledge the financial support of Carl Zeiss Microscopy, Hamamatsu Corporation, LaVision BioTec, TissueVision, Inc. and Chroma Technology Corp. Nature Methods carries sole responsibility for all editorial content and peer review.
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Pastrana, E. Focus on Mapping the Brain. Nat Methods 10, 481 (2013). https://doi.org/10.1038/nmeth.2509
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