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OpenSpinMicroscopy: an open-source integrated microscopy platform

Nature Methods volume 10, pages 599–600 (2013)Cite this article

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Figure 1: Multimodality imaging with OpenSpinMicroscopy.

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Acknowledgements

We thank C. Gonzalez (Institute for Research in Biomedicine Barcelona) for the use of his line of flies; M. Bettencourt-Dias and A. Jurberg (Instituto Gulbenkian de Ciência) for providing flies and mutant mouse embryos; and A.C. Borges (Centro de Estudos de Doenças Crónicas) for providing zebrafish embryos. E.J.G. acknowledges support from the Fundação para a Ciência e a Tecnologia grant SFRH/BPD/80717/2011. G.G.M. acknowledges the support of the Microscopy Unit of Faculdade de Ciências da Universidade de Lisboa.

Author information

Affiliations

  1. Instituto Gulbenkian de Ciência, Oeiras, Portugal

    Emilio J Gualda, Tiago Vale, Pedro Almada, José A Feijó & Nuno Moreno

  2. Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal

    José A Feijó

  3. Centro de Biologia Ambiental, Faculdade de Ciências da Universidade de Lisboa, Lisbon, Portugal

    Gabriel G Martins

Authors
  1. Emilio J Gualda
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  2. Tiago Vale
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  3. Pedro Almada
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  4. José A Feijó
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  5. Gabriel G Martins
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  6. Nuno Moreno
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Corresponding author

Correspondence to Emilio J Gualda.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–6, Supplementary Table 1 and Supplementary Notes 1–3 (PDF 862 kb)

DSLM time-lapse recording

Maximum-intensity projection of the development over 8 h of a zebrafish embryo (Tg(β-actin:HRAS-EGFP)) expressing GFP acquired with the OpenSpinMicroscopy plug-in. (MOV 4808 kb)

Multiple view fusion

Volume reconstruction obtained after the fusion of eight different views of a transgenic mouse embryo (FVB/N.Tie II-GFP) expressing GFP. (MOV 1498 kb)

OPT reconstruction of E14.5 mouse embryo

Series of sagittal sections of an E14.5 mouse embryo obtained after back-projection reconstruction of an OPT dataset into axial sections. The OPT dataset represents green autofluorescence and was acquired using the dedicated OPT setup with a 1× magnification lens. (MOV 1264 kb)

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Gualda, E., Vale, T., Almada, P. et al. OpenSpinMicroscopy: an open-source integrated microscopy platform. Nat Methods 10, 599–600 (2013). https://doi.org/10.1038/nmeth.2508

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