Transfer of genomes into yeast facilitates genome engineering for genetically intractable organisms, but this process has been hampered by the need for cumbersome isolation of intact genomes before transfer. Here we demonstrate direct cell-to-cell transfer of bacterial genomes as large as 1.8 megabases (Mb) into yeast under conditions that promote cell fusion. Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted in the enhancement of genome transfer.
This is a preview of subscription content, access via your institution
Open Access articles citing this article.
Scientific Reports Open Access 21 May 2018
Nature Communications Open Access 26 October 2016
Journal of Biological Engineering Open Access 10 December 2013
Subscribe to Journal
Get full journal access for 1 year
only $8.25 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Gibson, D.G. et al. Science 319, 1215–1220 (2008).
Noskov, V.N., Segall-Shapiro, T.H. & Chuang, R.Y. Nucleic Acids Res. 38, 2570–2576 (2010).
Suzuki, Y. et al. Nat. Methods 8, 159–164 (2011).
Hinnen, A., Hicks, J.B. & Fink, G.R. Proc. Natl. Acad. Sci. USA 75, 1929–1933 (1978).
Ito, H., Fukuda, Y., Murata, K. & Kimura, A. J. Bacteriol. 153, 163–168 (1983).
Kouprina, N. & Larionov, V. Nat. Protoc. 3, 371–377 (2008).
Gibson, D.G. et al. Science 329, 52–56 (2010).
Gibson, D.G. et al. Proc. Natl. Acad. Sci. USA 105, 20404–20409 (2008).
Benders, G.A. et al. Nucleic Acids Res. 38, 2558–2569 (2010).
Lartigue, C. et al. Science 325, 1693–1696 (2009).
Karas, B.J., Tagwerker, C., Yonemoto, I.T., Hutchison, C.A. III & Smith, H.O. ACS Synthetic Biol. 1, 22–28 (2011).
Tagwerker, C. et al. Nucleic Acids Res. 40, 10375–10383 (2012).
Noskov, V.N. et al. ACS Synthetic Biol. 1, 267–273 (2012).
Ushijima, S., Nakadai, T. & Uchida, K. Agric. Biol. Chem. 55, 129–136 (1991).
Maehara, T., Itaya, M., Ogura, M. & Tanaka, T. FEMS Microbiol. Lett. 325, 49–55 (2011).
Tarshis, M., Salman, M. & Rottem, S. FEMS Microbiol. Lett. 66, 67–71 (1991).
van Solingen, P. & van der Plaat, J.B. J. Bacteriol. 130, 946–947 (1977).
Gyuris, J. & Duda, E.G. Mol. Cell Biol. 6, 3295–3297 (1986).
Larionov, V., Kouprina, N., Solomon, G., Barrett, J.C. & Resnick, M.A. Proc. Natl. Acad. Sci. USA 94, 7384–7387 (1997).
Lee, D.H., Miles, R.J. & Inal, J.R. Epidemiol. Infect. 98, 361–368 (1987).
Tully, J.G., Rose, D.L., Whitcomb, R.F. & Wenzel, R.P. J. Infect. Dis. 139, 478–482 (1979).
Poje, G. & Redfield, R.J. Methods Mol. Med. 71, 51–56 (2003).
We thank J. Firstenhaupt for preparing Figure 2a. This work was supported by Synthetic Genomics, Inc. B.J.K. was supported by the National Science and Engineering Research Council of Canada Postdoctoral Fellowships Program and by Synthetic Genomics, Inc. Y.S. was supported by the US Defense Advanced Research Projects Agency contract N66001-12-C-4039.
J.C.V. is chief executive officer and co-chief scientific officer, H.O.S. is co-chief scientific officer and a member of the board of directors, C.A.H. is chairman of the scientific advisory board, and D.G.G. is a vice president of Synthetic Genomics, Inc. All four of these authors and the J. Craig Venter Institute hold shares of this company.
About this article
Cite this article
Karas, B., Jablanovic, J., Sun, L. et al. Direct transfer of whole genomes from bacteria to yeast. Nat Methods 10, 410–412 (2013). https://doi.org/10.1038/nmeth.2433
This article is cited by
Scientific Reports (2018)
Nature Communications (2016)
Chromosome Research (2015)
Nature Methods (2014)