Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Reproducibility restored—on toward the human interactome

A two-laboratory study of the reproducibility of affinity purification–mass spectrometry shows that a standardized protocol results in highly reproducible interactome data.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Sources of variation in AP-MS experiments.

Katie Vicari

References

  1. Varjosalo, M. et al. Nat. Methods 10, 307–314 (2013).

    Article  CAS  Google Scholar 

  2. Braun, P. Proteomics 12, 1499–1518 (2012).

    Article  CAS  Google Scholar 

  3. Vidal, M., Cusick, M.E. & Barabasi, A.-L. Cell 144, 986–998 (2011).

    Article  CAS  Google Scholar 

  4. Gavin, A.C. et al. Nature 440, 631–636 (2006).

    Article  CAS  Google Scholar 

  5. Krogan, N.J. et al. Nature 440, 637–643 (2006).

    Article  CAS  Google Scholar 

  6. Collins, S.R. et al. Mol. Cell Proteomics 6, 439–450 (2007).

    Article  CAS  Google Scholar 

  7. Ito, T. et al. Proc. Natl. Acad. Sci. USA 98, 4569–4574 (2001).

    Article  CAS  Google Scholar 

  8. Braun, P. et al. Nat. Methods 6, 91–97 (2009).

    Article  CAS  Google Scholar 

  9. Cusick, M.E. et al. Nat. Methods 6, 39–46 (2009).

    Article  CAS  Google Scholar 

  10. Choi, H. et al. Nat. Methods 8, 70–73 (2011).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work is supported by the Collaborative Research Centre (Sonderforschungsbereich) SFB924, funded by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Pascal Braun.

Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Braun, P. Reproducibility restored—on toward the human interactome. Nat Methods 10, 301–303 (2013). https://doi.org/10.1038/nmeth.2412

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.2412

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing