We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
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We thank A.J. Bureta for help with figure illustrations, R. Manis for critical proofreading and A. Gasch (University of Wisconsin-Madison) for assistance in the culturing of yeast cells. This work was supported by US National Institutes of Health (NIH) grant R01 GM080148 to J.J.C. A.E.M. gratefully acknowledges support from an NIH-funded Genomic Sciences Training Program (5T32HG002760). This work was also supported by a Searle Scholars Award and Shaw Scientist award to D.J.P. and a National Science Foundation graduate fellowship and NIH training grant 5T32GM007215-37 to A.J.S.
A.S.H. and J.J.C. are co-inventors on a patent application (US 13/660677) related in part to the material presented here.
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Hebert, A., Merrill, A., Bailey, D. et al. Neutron-encoded mass signatures for multiplexed proteome quantification. Nat Methods 10, 332–334 (2013). https://doi.org/10.1038/nmeth.2378
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