Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Accelerating 3B single-molecule super-resolution microscopy with cloud computing

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: 3B analysis using Amazon EC2.


  1. Cox, S. et al. Nat. Methods 9, 195–200 (2012).

    CAS  Article  Google Scholar 

  2. Betzig, E. et al. Science 313, 1642–1645 (2006).

    CAS  Article  Google Scholar 

  3. Patterson, G., Davidson, M., Manley, S. & Lippincott-Schwartz, J. Annu. Rev. Phys. Chem. 61, 345–367 (2010).

    CAS  Article  Google Scholar 

  4. Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    CAS  Article  Google Scholar 

  5. Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).

    CAS  Article  Google Scholar 

  6. Dertinger, T., Colyer, R., Iyer, G., Weiss, S. & Enderlein, J. Proc. Natl. Acad. Sci. USA 106, 22287–22292 (2009).

    CAS  Article  Google Scholar 

  7. Burnette, D.T., Sengupta, P., Dai, Y., Lippincott-Schwartz, J. & Kachar, B. Proc. Natl. Acad. Sci. USA 108, 21081–21086 (2011).

    CAS  Article  Google Scholar 

Download references


We thank E. Rotsen for helping us install the 3B analysis software. This research is supported by the Waitt Foundation.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Hu Cang.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Demonstration of Amazon EC2 for 3B analysis

This brief video demonstrates the procedures of setting up an Amazon EC2 account, launching 3B calculation instances using the AMI and bash scripts we supply, visualizing reconstruction results and terminating EC2 instances using a Macintosh computer. (MOV 16669 kb)

Supplementary Software

The file contains the bash and MATLAB scripts that we prepared to run the 3B analysis on the Amazon EC2 cloud. (ZIP 706 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hu, Y., Nan, X., Sengupta, P. et al. Accelerating 3B single-molecule super-resolution microscopy with cloud computing. Nat Methods 10, 96–97 (2013).

Download citation

  • Published:

  • Issue Date:

  • DOI:

Further reading


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing